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Like base excision repair, nucleotide excision repair is present in all organisms In yeast, approximately twelve genes are involved, many in what is called the RAD3 group In human beings, twenty- ve proteins are involved; they remove twenty-seven to twenty-nine nucleotides, as compared to twelve to thirteen in E coli Transcription and nucleotide excision repair are linked in eukaryotesTranscription factor TFIIH (see chapter 11) is involved in repair of UV damage; it has helicase activity and is found in both processes Since it has been shown that genes that are actively being transcribed are preferentially repaired, we can now envision a model in which transcription, when blocked by a DNA lesion like a thymine dimer, signals the formation of a repair complex, using TFIIH in both processes In prokaryotes, RNA polymerase dissociates from the DNA in this circumstance, losing the nascent transcript.

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Saturation VBB = 5 V; VCE = 644 V 374%; because RE provides a negative feedback action that will keep IC and IE almost constant Saturation; 85 mA 159 mW The MOSFET is in the ohmic region Choosing the smaller value of vGS , RS = 207 k , vGS = 286 V, iD = 0586 mA The answer is not unique: Selecting the larger gate voltage, we nd RS = 115 k Approximately 400 200 Approximately 25 mA iD = 389 mA; vDS = 1057 V; vGS = 058 V RS = 634 ; RD = 6366 k

Fig 412: Bondless diamond grinding of chip packaging on the Pentium III IC chip revealing defects such as

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This would be inef cient in eukaryotes, whose genes are much longer and more expensive to transcribe; for example, the human dystrophin gene, defective in the disease Duchenne muscular dystrophy, is 24 million bases long and takes almost eight hours to transcribe We believe that eukaryotic RNA polymerase II backs up when stalled at a DNA lesion and continues after the lesion is repaired, without losing the transcript Much active research is going on in this area In human beings, the autosomal recessive trait xeroderma pigmentosum is caused by an inability to repair thymine dimerization induced by UV light Persons with this trait freckle heavily when exposed to the UV rays of the sun, and they have a high incidence of skin cancer.

magnitudes of the voltages across the emitter-base and collector-base junctions are 065 and 73 V Find a VCE b IC c The total power dissipated in the transistor, de ned here as P = VCE IC + VBE IB

07 V _ _ (c)

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There are seven complementation groups (loci XPA-XPG) whose protein products are involved in the rst steps of nucleotide excision repair and whose defects cause xeroderma pigmentosum in human beings One of them, XPD, is a component of TFIIH Excision repair triggered by mismatches is referred to as mismatch repair, which encompasses about 99% of all DNA repairs As DNA polymerase replicates DNA, some errors are made that the proofreading polymerase does not correct For example, a template G can be paired with a T rather than a C in the progeny strandThe GT base pair does not t correctly in the DNA duplex The mismatch repair system, which follows behind the replicating fork, recognizes this problem This system, whose members in E coli are speci ed by the mutH, mutL, mutS, and mutU genes, is responsible for the removal of the incorrect base by an excision repair process.

+ (d)

operating point of the transistor Assume a 06-V offset voltage and = 150 In what region is the transistor

(The genes are called mut for mutator because mutations of these genes cause high levels of spontaneous mutation in the cells The mutU gene is also known as uvrD) The mismatch repair enzymes initiate the removal of the incorrect base by nicking the DNA strand on one side of the mismatch You might wonder how the mismatch repair system recognizes the progeny, rather than the template, base as.

(a) tear of Cu pads and (b) eccentric viaducts [28]

transistors: a npn, VBE = 08 V, VCE = 04 V b npn, VCB = 14 V, VCE = 21 V c pnp, VCB = 09 V, VCE = 04 V d npn, VBE = 12 V, VCB = 06 V

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