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Analysis: The information regarding the breakdown voltage, VB , is useful to select the drain supply voltage The drain supply must be less than the breakdown voltage to prevent device failure; VDD = 24 V is a reasonable choice The resistance RG serves the purpose of tying the gate to ground This is usually accomplished with a large resistance Any leakage current would be only of the order of nanoamperes, so the gate would be at most a few mV above ground Let s choose RG = 1 M Using the universal equation for the saturation region from Table 92, we write:

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a uracil-DNA glycosylase enzyme removes a uracil (red) from DNA. An AP (apurinic-apyrimidinic) endonuclease nicks the DNA on the 5 side of the base-free site. Between 80 and 90% of the time, a DNA polymerase will replace the single nucleotide (green); an exonuclease, lyase, or phosphodiesterase will remove the base-free nucleotide. The nal nick is sealed with DNA ligase. Between 10 and 20% of the time, the DNA polymerase will extend polymerization beyond the single nucleotide. In those cases, an exonuclease and DNA ligase nish the repair. (From T. Lindahl, The Croonian Lecture, 1996:

500X

Knowing the desired drain current, we can expand the above equation to obtain: IDSS 2 IDSS v +2 vGS + 2 iDQ VP iDQ VP GS IDSS 1 =0 iDQ

Endogenous Damage to DNA, Philosophical Transactions of the Royal Society of London, B351, pp. 1529 1538, gure 6, 1996.)

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Since the JFET is in the active region only if vGS > VP , the only acceptable solution is vGSQ = 055 V To obtain the desired value of vGSQ we must choose an appropriate value of RS Remember that we know the value of the quiescent drain current (we desire 4 mA) Thus, we desire vGSQ = vG vS = 055 V, and since vG = 0: vS = 055 = iDQ RS or RS = 055 = 1375 0004

Now we can write the drain circuit equation to determine the appropriate value of RD to ensure that vDS = 10 V: VDD = RD iD + vDS + RS iD or 24 = 0004RD + 10 + 055 from which equation RD can be computed to be approximately 336 k

The McGraw Hill Companies, 2001

Comments: The value of source and drain resistance computed in the above example are not standard resistor values (see 2, Table 21); engineering practice would require that the nearest available standard values be used: RS = 150 , RD = 333 k

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944(b)

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UvrA2UvrB locates lesion 5 T G C G C G A A C T=T C G G A A A C G C G C T T G A A G C C T T 3 UvrA2 dissociates UvrC attaches 5 3

Schematics, Diagrams, Circuits, and Given Data: VP = 3 V; IDSS = 6 mA; RS = 1 k Assumptions: Use the JFET equations of Table 92 Analysis: We have already seen in Example 912 that a JFET can be biased in the

T G C G C G A A C T T C G G A A A C G C G C T T G A A G C C T T Figure 12.31 Nucleotide excision repair. A lesion in DNA (a thymine dimer) is located by a protein made of two copies of UvrA and one of UvrB. Then, the UvrA subunits detach, and UvrC attaches on the 5 side of the lesion. UvrB nicks the DNA on the 3 side and UvrC on the 5 side of the lesion; UvrD helicase unwinds the oligonucleotide containing the lesion (red). DNA polymerase I and DNA ligase then repair the patch (green).

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convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp

convert tiff to pdf c# itextsharp